Distinção de espécies e estádios de maturação de sementes de Comanthera spp. por análise de imagem e citometria de fluxo

Comanthera spp. are perennial and commonly used for ornamentation, of high economic and social value, this fact hascompromised the conservation of populations because they are harvested before the maturation of the seeds. The firstexperiment was carried to verify the efficiency of the image analysis system in the distinction of two Comanthera speciesat different development stages of inflorescence, as well as the germination potential of the seeds. The second assaywas carried out to estimate the nuclear DNA content in the species distinction, establish the appropriate buffer for theisolation of interphase nuclei and investigate the feasibility of using different parts of the plant for analysis by flowcytometry. It was verified that the image analysis system has the potential to differentiate species and developmentstages of inflorescence of sempre-vivas. The C. elegans species has higher germination values in stages 4 and 5, presentinga head with opening higher than 60%. Leaves, roots and seeds of C. bisulcata, C. elegans and C. nitida can be used forquantification of nuclear DNA by cytometry. The Marie buffer is indicated for the extraction of DNA from leaf and roottissues and the LB01 buffer for seeds.