Isolation, identification, and bioactivity of steroids isolates from Hydrilla verticillata petroleum ether fraction

Hydrilla verticillata contains some active compounds that potential as an antioxidant, antibacterial, anticancer, antimicrobial and antitumor. One of the active compounds in Hydrilla verticillata is steroids. This research aimed to isolate, to identify and to determine the toxicity and antioxidant activity of steroid compounds in petroleum ether (PE) fraction of Hydrilla verticillata. Hydrilla verticillata biomass powder was extracted by maceration using ethanol solvent. The ethanol extract was hydrolyzed with 2 N of hydrochloric acid and then partitioned with petroleum ether solvent. The steroid compounds from petroleum ether fraction were separated with Preparative Thin Layer Chromatography (TLC) and Column Chromatography. The steroid isolates were identified by UV-Vis and FTIR spectrophotometer. The toxicity level and antioxidant assay of steroid isolates were determined by BSLT and DPPH method, respectively. The result of the study showed that extraction through maceration produced 4.54% yield, whereas the product yield of the partition using petroleum ether was 65.41%. The steroid isolates from TLC and Column Chromatography separation has toxicity and antioxidant properties. The LC50 value of steroid TLC isolate was 1.41 and 12.2 ppm. The LC5o value of steroid Column Chromatography isolates (H2, H4 and H10) were 4.32, 8.24 and 10.35 ppm. The EC50 value of H2 Column Chromatography isolate was 23.00 ppm.