CNF/AgNP/chitosan Film With Antimicrobial and Antihemolytic Properties for Wound Healing

Cellulose nanofibers (CNF), chitosan, and silver nanoparticles (AgNP) are widely used to enhance the active functions and antibacterial properties in wound dressing. This study was conducted to prepare CNF/AgNP/chitosan incorporation by straight incorporated method and its antimicrobial activity. CNF were isolated from oil palm empty fruit bunches (OPEFB) by pulping method and acid hydrolysis. AgNP were synthesized by green synthesis method. The wound dressing was produced by mixing 10% CNF solution in LiCl/DMAc and AgNP-chitosan in a glass plate with various variations of CNF/AgNP/chitosan, i.e. 100:0, 80:20, 60:40, and 50:50. UV-visible and TEM analyses were used to confirm the formation of AgNP and CNF in nanoscale. The results showed the particles with an absorption of 435 nm and spherical shapes. Based on the calculation by using imageJ analysis, the diameters of CNF were about 50 nm and lengths of several micrometers. FTIR was used to analyze the chemical bonding of AgNP/chitosan and the incorporated the AgNP/chitosan in CNF. From XRD analysis, the presence of AgNP did not affect the crystallinity of CNF. SEM images showed that the addition of AgNP resulted in the stretching of CNF pores on the pads. Thermal degradation of the film increased with the addition of AgNP/chitosan by up to 40%. Antimicrobial test and hemocompatibility test were shown that the formed film of CNF/AgNP/chitosan successfully inhibit bacterial growth and classified as non-hemolytic materials, thus can be further studied its potential as wound dressing.