In silico and in vivo approaches to recombinant multi-epitope immunogen of GroEL provides efficient cross protection against S. Typhimurium, S. flexneri, and S. dysenteriae

•Developing an universal immunogen against bacterial pathogens is important.•The study aims to design of a GroEL multi-epitope as a universal immunogen.•Animals immunized with recombinant multi-epitope.•The protein has immunogenicity and cross protection against Shigella and Sallmonella.