Quantitative Proteomics of Polarised Macrophages Derived from Induced Pluripotent Stem Cells

Macrophages (M-phi) are highly heterogenous and versatile innate immune cells involved in homeostatic and immune responses. Activated M-phi can exist in two extreme phenotypes: pro-inflammatory (M1) M-phi and anti-inflammatory (M2) M-phi. These phenotypes can be recapitulated in vitro by using ligands of toll-like receptors (TLRs) and cytokines such as IFN gamma and IL-4. In recent years, human induced pluripotent stem cells (iPSC)-derived M-phi have gained major attention, as they are functionally similar to human monocyte-derived M-phi and are receptive to genome editing. In this study, we polarised iPSC-derived M-phi to M1 or M2 and analysed their proteome and secretome profiles using quantitative proteomics. These comprehensive proteomic data sets provide new insights into functions of polarised M-phi.