Single-nucleotide resolution of N⁶-adenine methylation sites in DNA and RNA by nitrite sequencing

Yasaman Mahdavi-Amiri,Kimberley Chung Kim Chung,Ryan Hili

Chemical Science（2021）

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摘要

Chemoselective deamination of adenine in the presence of N6-methyladenine using nitrite enables single-nucleotide resolution sequencing of N6-adenine methylation sites in DNA and RNA.

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Single-nucleotide resolution of N6-adenine methylation sites in DNA and RNA by nitrite sequencing

Single-nucleotide resolution of N⁶-adenine methylation sites in DNA and RNA by nitrite sequencing