Environmental DNA (eDNA) extraction using Qiagen DNeasy Blood and Tissue Kit v2

Nucleic acids extraction from the filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Thomsen et al (2012), are used by the Monterey Bay Aquarium Research Institute (MBARI) for Monterey Bay, CA samples (MB). All MB samples were extracted in the Chavez Laboratory at the Monterey Bay Aquarium Research Institute, Moss Landing California. This work was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a Demonstration Marine Biodiversity Observation Network (MBON)’ funded under the National Ocean Partnership Program (NOPP RFP NOAA-NOS-IOOS-2014-2003803 in partnership between NOAA, BOEM, and NASA), and the U.S. Integrated Ocean Observing System (IOOS) Program Office. Thomsen, P. F., Kielgast, J., Iversen, L. L. N., Møller, P. R., Rasmussen, M. & Willerslev, E., 2012, Detection of a Diverse Marine Fish Fauna Using Environmental DNA from Seawater Samples. Plos One, 7(8),e41732. Qiagen Inc., July 2006 DNeasy Blood and Tissue Handbook; available online from Qiagen Inc§ . Qiagen, Inc., June 2012 Qiamp DNA Mini and Blood Mini Handbook, available online from Qiagen, Inc.