Intelligent image-activated cell sorting: principles and applications (Conference Presentation)

I present a newly developed machine intelligence technology called “Intelligent Image-Activated Cell Sorting” [Cell 175, 1 (2018)] that achieves real-time fluorescence-image-based intelligent cell sorting at a high throughput of ~100 events per second. This technology builds on a unique integration of high-throughput cell microscopy, focusing, and sorting techniques on a unique software-hardware platform and hence performs fully automated operation for data acquisition, data processing, decision making, and actuation. Also, I introduce the technology’s broad utility to addressing a fundamental biological question that cannot be answered by conventional technologies – how molecular architectures of cells are connected with their physiological functions.