Angiotensin Converting Enzyme Inhibition with Antiradicalar Activity of Thornback Ray Gelatin Hydrolysate: Determination and Separation of Peptide Fractions

Thornback Ray Skin Gelatin Hydrolysate with an hydrolysis degree of 9.23% was prepared by treatment with Alcalase (TRGH-Alcalase). The hydrolysate was found to exhibit ACE inhibitory activity and 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) scavenging activity with an IC50 values of 0.92 and > 5 mg/mL, respectively. The molecular weight distribution of the hydrolysate showed the abundance of small-sized peptides with average molecular weight below 1000 Da (57%) contrary to the untreated gelatin. The hydrolysate and the gelatin did not show hemolytic activity at a concentration of 2 mg/ml.