Effects of SNPs in POU1F1 promoter on growth traits in largemouth bass(Micropterus salmoides)

In the present study,the promoter sequence of largemouth bass POU1F1 was cloned which is 1 629 bp in length,predicted the existence of four octamer transcription factor 1(Oct-1)binding sites,one homeobox transcription factors sites,two cAMP-responsive element binding proteins sites and a TATA box.Using directly sequencing,two nucleotides mutations were identified which were located at the-18 and-183 sites,and only two haplotypes were identified(haplotype A and haplotype B).The different genotype individuals and the mutant alleles in stochastic population(126 individuals)were examined.The frequency of A haplotype and B haplotype was 0.246 and 0.754 respectively.The population correlation analysis showed that the AA and AB genotypes have significantly correlated with BB genotype individuals' weight,body length,full length,body depth and body width(P0.05),but there is no significant correlation between AA genotype individuals and AB genotype individuals.Identified growth traits markers for growth traits could be implemented through marker-assisted selection(MAS),with the aim of producing rapidly growing largemouth bass.