High-throughput analysis of the steroid profile in placental cell cultures to evaluate endocrine disrupting effects of contaminant exposure

Júlia Mestres,Elisabet Pérez-Albaladejo,Cinta Porte,Cristina Postigo

Journal of Chromatography A（2022）

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摘要

•Analysis of steroid profile in JEG-3 cell culture medium to assess reprotoxic effects.•Trace analysis of steroids with turbulent flow chromatography and LC-MS/MS.•High throughput, safe and green alternative to radioactive measurements.•Application of this method revealed an estrogenic effect of iodoacetic acid.

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关键词

Steroid profiling,Disinfection byproducts,Turbulent flow chromatography,Placental JEG-3 cells,Sex hormones,Reprotoxicity

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