Superphosphate, biochar, and a microbial inoculum regulate phytotoxicity and humification during chicken manure composting

The germination index (GI) is the best index for evaluating compost phytotoxicity and maturity. In order to improve GI and reduce phytotoxicity of chicken manure compost, superphosphate, biochar, and a microbial inoculum were added in this study. Maturity indices (pH, electrical conductivity, and GI), water-soluble ion, organic matter, humic acid, humic precursor contents, and the bacteria community were analyzed during the experiment. NH4+, volatile fatty acids, and humic acid strongly affected the GI, which increased as the humic acid content increased and the volatile fatty acid and NH4+ contents decreased. The three additives affected compost maturity differently. Adding biochar decreased microbial diversity and complexity, but improved the GI mainly by affecting abiotic factors. Adding the microbial inoculum increased biotic activity and promoted humus and precursor formation. Superphosphate activated core functional bacteria and increased bacterial diversity and complexity, and 16 genera and 2 phyla (Gemmatimonadota and Chloroflexi) were found only in this composting pile. Superphosphate markedly accelerated humification and decreased the salt (NH4+ and NO3−) and heavy metal ion (Cu2+, Cd2+, Cr3+) contents, forming stable substances to reduce the key phytotoxic matters, which in turn decreased the compost phytotoxicity and improved the GI. These results provide a new sight for promoting maturity by functional material regulation in composting.