Assessment of machine perfusion conditions for the donation after circulatory death heart preservation

Background Donation after circulatory death (DCD) hearts requires machine perfusion preservation, the conditions of which are not well defined. Methods To achieve this, rat hearts were procured following a DCD or control beating-heart donation (CBD) model, and perfused for 60 min with one of three machine perfusion solutions-St. Thomas (ST), University of Wisconsin (UW), or Polyethylene Glycol-20k (PEG)-at one of two temperatures, 4 degrees C or 15 degrees C. At 15-min intervals, perfusion pressure was measured as a marker of vascular resistance. Colored microspheres were added to capture the distribution of perfusate into the metabolically active sub-endocardium, and the eluate was collected for troponin assays. Analyses compared groups using Wilcoxon rank-sum and ANOVA. Results Perfusion pressure was significantly higher for DCD than CBD hearts at 15 degrees C regardless of solutions. The lowest rise in perfusion pressure over time was observed with PEG at 15 degrees C. Except for PEG at 15 degrees C, ST and UW solutions at 4 or 15 degrees C had decreased sub-endocardial perfusion in DCD hearts. Troponin release from DCD hearts with UW and PEG solutions was comparable to CBD hearts but was significantly higher with ST solution at 15 degrees C. Conclusions Optimal preservation conditions for DCD hearts were observed with PEG machine perfusion solution at 15 degrees C.