Methods paper: Performance characteristics of novel assays for circulating levels of proglucagon-derived peptides and validation in a placebo controlled cross-over randomized clinical trial

•Sequences of PGDPs are overlapping and their low circulating levels make their measurement difficult.•Novel assays can perform an accurate, precise, and specific measurement of PGDPs.•Regulation of glucose homeostasis by coffee may occur through altering circulating levels of PGDPs.