In Situ HiC.

Methods in molecular biology (Clifton, N.J.)（2022）

引用 1|浏览6

暂无评分

摘要

In situ HiC uses the relative frequency of DNA-DNA ligation events to reconstruct the three-dimensional architecture of a genome. As such, restriction enzyme digested ends of genomic DNA within fixed nuclei are tagged with biotinylated dNTPs. DNA-DNA ligation events generated via proximity ligation are then captured, amplified and next generation sequenced to determine their linear genomic position, but also their three-dimensional relationship. Here, we describe these steps in detail.

查看译文

关键词

Chromatin architecture,Chromosome conformation capture,Genome organization,In situ HiC

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要