A hybrid oxygen-generating wound dressing based on chitosan thermosensitive hydrogel and decellularized amniotic membrane

Amniotic membrane (AM) has been utilized as a wound dressing extensively. Given the importance of oxygen in wound healing, here we have reported the fabrication and characterization of an oxygen-generating wound dressing based on AM. This construct was composed of H2O2-loaded polylactic acid (PLA) microparticles embedded within a chitosan/beta-glycerophosphate (beta-GP) thermosensitive hydrogel covered with a layer of decellularized human-AM. The microparticles had a diameter of 4.48 +/- 1.8 mu m, an encapsulation efficiency of 44.172 +/- 4.49%, and generated oxygen for at least 7 days. The hybrid construct was formed at 32.4 +/- 2 degrees C, had a porous structure (84.69 +/- 8.34%) with a pore size of 46.72 +/- 26.21 tint. The hydrogel/dAM extract was nontoxic after 7 days based on our MTT results, and the final composite supported cell growth and adhesion. This sample had the most negligible blood cell adhesion with less than 5% hemolysis. Our results indicate the proposed structure's desirable biological, chemical, and physical properties as an active wound dressing.