Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)

Kelkar Vipul Ganesh,Deepu Mathew,Rehna Augustine,Soni K B,Swapna Alex,Shylaja M R,Anita Cherian K

Journal of Virological Methods（2022）

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摘要

•Interference with viral βC1 ORF offers YVMV resistance in transgenic okra lines.•ihpRNA cassette with sense and antisense strands was cloned in pRNAi-LIC vector.•Ligation independent cloning is an easier, PCR based strategy.•Agrobacterium mediated in planta transformation is an efficient strategy in okra.•Artificial screening with infected vectors in containment has proven the resistance.

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关键词

Abelmoschus esculentus,Begomovirus,βC1,Genetic engineering,In planta transformation,RNAi,YVMV

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