Expression, purification and microscopic characterization of transmembrane BAX Inhibitor-1 motif containing protein 5.

Transmembrane bax inhibitor-1 motif containing protein 5 (TMBIM5) is located on the inner membrane of mitochondria and is widely expressed in tissues but less frequently in the intestine and thymus. TMBIM5 affects mitochondrial cristae organization and is associated with Parkinson's disease. Here, we present the first report about expression, purification and the 2D classification projections derived from negatively stained electron micrographs of recombinant H. sapiens TMBIM5 (hTMBIM5). The described methods and results will support further structural and functional study of hTMBIM5.