Fine mapping and cloning of the major seed protein quantitative trait loci on soybean chromosome 20

Soybean is the most important source of protein meal worldwide and the quantitative trait loci (QTL) cqSeed protein-003 on chromosome 20 exerts the greatest additive effect of any protein QTL mapped in the crop. Through genetic mapping and candidate gene downregulation, we identified that an insertion/deletion variant in Glyma.20G85100 is the likely gene that underlies this important QTL.