Structure-Guided Design, Synthesis, and Evaluation of 1-Indanone and 1,3-Indandione Derivatives as Ligands for Misfolded α-Synuclein Aggregates
semanticscholar(2021)
摘要
The development of imaging
agents for in vivo detection of alpha-synuclein (α-syn) pathologies faces
several challenges. A major gap in the field is the lack of diverse molecular
scaffolds with high affinity and selectivity to α-syn fibrils for in vitro
screening assays. Better in vitro
scaffolds can instruct the discovery of better in vivo agents. We report the
rational design, synthesis, and in vitro evaluation of a series of novel
1-indanone and 1,3-indandione derivatives from a Structure-Activity
Relationship (SAR) study centered on some existing α-syn fibril binding
ligands. Our results from fibril saturation binding experiments show that two
of the lead candidates bind α-syn fibrils with binding constants (Kd)
of 9.0 and 18.8 nM, respectively, and selectivity of greater than 10x for α-syn
fibrils compared with amyloid-β (Aβ) fibrils. Our results demonstrate that the
lead ligands avidly label all forms of α-syn on PD brain tissue sections, but
only the dense core of senile plaques in AD brain tissue, respectively. These
results are corroborated by ligand-antibody colocalization data from Syn211,
which shows immunoreactivity towards all forms of α-syn aggregates, and Syn303,
which displays preferential reactivity towards mature Lewy pathology. Our results reveal that 1-indanone derivatives
have desirable properties for the biological evaluation of α-synucleinopathies.
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