Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR
semanticscholar(2019)
摘要
Phospholipid quantification by 31P NMR and lipid separation and
detection by LC-MS/MS. Fatty acid quantification using scripted
reconstruction of elution profile for each fatty acid associated with
each lipid species. The combination of NMR and and MS data is
complementary and allows better quantification and more complete
description than each approach would allow by itself. Datasets exists
for both whole-cell and plasma membrane fraction, and we also describe
and document the extraction techniques.
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