Methods of “ Thermal proteome profiling for unbiased identification of direct and indirect drug targets “

 Liquid nitrogen (use any local provider)  K562 cell line (ATCC, cat. no. CCL-243)  HDAC inhibitor Panobinostat (Santa Cruz Biotechnology , cat. no. SC-208148)  RPMI1640 medium (Life Technologies, cat. no. 21875-034)  FCS (Life Technologies, cat. no. 10270-106)  DPBS without calcium chloride/ magnesium chloride (Life Technologies, cat. no. 14190-094)  DMSO waterfree (Sigma-Aldrich, cat. no. 41647)  cOmplete, EDTA-free protease inhibitors (Roche, cat. no. 11873580001)  Dimethyl sulfoxide DMSO (Fluka , cat. no. 01934-1L)