Identification of differentially expressed genes by cdna-aflp technique in response to salinity stress in bread wheat

semanticscholar(2020)

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摘要
Wheat may be a quality cereal crop on the world, notably in an Asian country, with facile money price is sensitive to salt stress, which is a tangible associate example of dissent consequence on crop yields. The direct of this work was to review three wheat genotypes differing in their come to salinity stress and institution of genetic mechanisms entangled reciprocally to salt stress in grain filling stage for the first time at the molecular levels under mRNA populations by cDNA-AFLP technique. The institution of those genes within the future can offer an appropriate basis for the assembly of changed and saline tolerant cultivars. In our scrutiny, we tend to pick out many salt-stress-responsive transcripts derived fragments (TDF) that were seasoned by RT-PCR. Most of the transcripts single out here, utilizing the essential native alignment search tool (BLAST) output, were genes be a part of distinct occupation classes delineate to destructive metabolism, potency, cellular biogenesis, cell definition, a signal for specific sensors, organic phenomenon regulation, supermolecule debasement, and transfer. The impact demonstrates that cDNA-AFLP could be a constant technique the procedure of salt stress tolerance genes in Triticum aestivum would be very fruitful for the breeding and biotechnology of salt tolerance heterogeneity in alternative Poaceae families additionally as barley and rice.
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