Application of proline and mannitol on lepidium sativum l. under abiotic stress condition (heavy metal stress)

The impact of proline and mannitol to heavy metal stress on morphological, physiological and nutritional analysis were studied in Lepidium sativum L. Morphological parameters (shoot and root length) physiological parameters (carbohydrate, protein, proline and relative water content) nutritional analysis (sodium and potassium) were determined after 45 and 90 days. Exogenous application of proline and mannitol 50μg/l and 100μg/l each were standardized and applied to heavy metal stress (CdSO4 50μM and 100μM). Proline and mannitol enhanced the morphological, physiological parameters and nutritional factors in stressed plant; these played a major protagonist in cellular osmotic adjustment. Present study indicating that the proline and mannitol play essential role to heavy metal stress in Lepidium sativum L. Therefore, it found that the plants are able to cope with heavy metal stress when exogenous proline and mannitol is applied.