Novel LNA probe-based assay for the A1 and A2 identification of β -casein gene in milk samples

Rodrigo Giglioti,Cintia Hiromi Okino,Bianca Tainá Azevedo,Gunta Gutmanis,Luciana Morita Katiki,Márcia Cristina de Sena Oliveira,Anibal Eugênio Vercesi Filho

Food Chemistry: Molecular Sciences（2021）

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摘要

•A novel LNA probe-based qPCR allowed the detection of A1 and A2 alleles from β -casein gene in bovine samples.•100% of agreement between results obtained by rHamp and LNA qPCR assays.•The limit of detection of A1 in A2 samples was 1% or 7.5 DNA copies.•This method is a highly sensitive and specific tool for detecting A1 and A2 alleles directly in milk.

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关键词

β-Casein,Sensitivity,Alleles,LNA-probe,real-time PCR

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