Vanquish Neo UHPLC system sets new performance standards for single-shot nanoLCMS bottom-up proteomics

Goal Demonstrate the superior performance of the Thermo ScientificTM VanquishTM Neo next-generation low-flow UHPLC system for nanoLCMS bottom-up proteome profiling when coupled to a Thermo ScientificTM Orbitrap ExplorisTM 480 mass spectrometer using the 75 μm I.D. × 75 cm Thermo ScientificTM EASY-SprayTM PepMap Neo column. Demonstrate system versatility and potential for new levels of proteomic depth coverage through the coupling of two 75 cm long nano-columns.