BBF RFC 110: BioBrick derived standard for in vitro and in vivo application of functional RNA

Functional RNA like siRNA, gRNA, aptamers and ribozymes provide a multitude of opportunities for the synthetic biologist to custom tailor new tools that can be used in vivo and in vitro. But as their structure and behavior depends on their base by base sequence, cloning their template DNA via restriction enzymes proves to be difficult. Cut sites like those used by the BioBrick RFC 10 Standard cannot be removed by mutagenesis as the exchange of one base might affect the function or performance of the functional RNA transcript. Therefore, the purpose of this RFC is to provide a standard to use sequence specific homology cloning to build pSB1C3 derived plasmids for storage and use of functional RNAs.