Analysis the role of miR-195 in Colorectal Adenocarcinoma Cancer

Email:M-hajari@scu.ac.ir; Mohamad.hajari@gmail.com Background: Colorectal cancer as a leading cause of cancer related death develops through multistep pathway. MicroRNAs (microRNAs) are a group of small noncoding RNAs that inhibit target mRNA translation. Previous studies have shown that abnormal expression of these genes is associated with colorectal cancer. It is also shown that abnormal expression of these genes is associated with colorectal cancer. miR-195 appears to be the major regulatory factor in various cancers including colorectal cancer. Further studies are needed to precisely determine the regulatory role of miR-195 in colorectal cancer. Thus, the present study was conducted to determine the expression level of miR-195 in colon adenocarcinoma and normal tissue specimens and to confirm this biomarker in tissue samples in Iran. Materials and Methods: In our case-control study, bioinformatic analyses were performed on gene expression and results were confirmed using experimental and in vitro analysis on clinical samples. Also, after RNA extraction and cDNA synthesis, gene expressionanalysis was done by using Real time PCR and then statistical analyses were performed. Results: The initiation of invasion and metastasis pathways may be due to decreased miR-195 expression. The qRT-PCR test results for the expression of genes showed that miR-195 expression decreased potentially in tumor tissues compared to adjacent normal tissues (p <0.00001). miR-195 is potentially associated with clinicopathologic features of tumors. Conclusion: The potential role of miR-195 in the development of colon cancer is shown.