Effect of mannitol on lepidium sativum l. under abiotic stress (water stress) condition

The effects of mannitol to water stress on physiological , nutritional, secondary metabolites and antioxidant capacity were investigated in Lepidium sativum L. Plants were grown under controlled temperature (25oC) and light conditions (16 hours light and 8 hours dark). Physiological parameters (carbohydrate, protein and proline) nutritional analysis (sodium and potassium) secondary metabolites (alkaloid and saponin) and antioxidant activity (ABTS and DPPH) were determined after 35, 75 and 110 days. Exogenous application of mannitol (50μg/l, 100μg/l, and 250μg/l) were standardized and applied to different water potential -0.01w MPa, -0.02w MPa and -0.03w MPa. Mannitol enhanced the physiological parameters, nutritional factors, secondary metabolites and antioxidant activity in stressed plant; it is play a major role in cellular osmotic adjustment. Present study indicating that the mannitol play pivotal role to water stress in Lepidium sativum L. Therefore, it found that the plants are able to cope with abiotic stress when exogenous mannitol is applied.