The synergistic antibacterial effect and inhibition of biofilm formation of nisin in combination with terpenes against Listeria monocytogenes

This study was to investigate the synergistic antibacterial effect and inhibition of biofilm formation of nisin in combination with terpenes (carvacrol, cinnamaldehyde, citral and thymol) against Listeria monocytogenes. The bactericidal ranking of terpenes combined with nisin was carvacrol > cinnamaldehyde, citral > thymol. The minimum inhibitory concentration (MIC) of nisin and carvacrol when used together was determined to be 0 center dot 1563 mg ml(-1) + 0 center dot 0195 mg ml(-1) (nisin at MIC/2 + carvacrol at MIC/16). The addition of nisin at MIC/2 + carvacrol at MIC/2 caused more decrease in membrane potential than carvacrol or nisin at MIC individually. The decrease rates of hlyA and plcA gene expressions caused by nisin at MIC/2 + carvacrol at MIC/2 were significantly higher than those caused by carvacrol or nisin at MIC individually (P < 0 center dot 05). Nisin combined with carvacrol showed the highest inhibition activity to the formation of L. monocytogenes biofilm on stainless steel and lettuce. The inhibition effect of nisin at MIC/2 + carvacrol at MIC/16 was significantly higher than that of nisin at MIC/2 and carvacrol at MIC/16 (P < 0 center dot 05).