The Therapeutic Effect of Shugan Xiehuo Formula in Female Rat Model with Central Precocious Puberty

Background: Central precocious puberty (CPP) severely affected children’s physical and mental health, which needs to be treated promptly and effectively.Objective: To research the therapeutic effect of Shugan Xiehuo Formula (SXF) on CPP.Methods: CPP female rat model was established and treated by leuprolide and different dose of SXF. Sex organs volume and index were measured. The ovary and uterus was experienced HE staining. Concentration of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and estradiol (E2) in peripheral blood was determined. Expression of gonadotropin-releasing hormone (GnRH), gonadotropin-releasing hormone receptor (GnRHR), estrogen receptor alpha (ERα) and G protein-coupled receptor 30 (GPR30) in hypophysis was investigated by qRT-PCR and Western Blot. GnRH expression in hypothalamus and GnRHR expression in ovary was detected by immunohistochemistry.Results: SXF reduced the volume of bilateral ovaries, uterus, hypothalamus and hypophysis in CPP female rat, and diminished the index of ovary, uterus, hypothalamus and hypophysis in CPP female rat (P < 0.05 or P < 0.01). SXF treatment inhibited follicles maturation and uterine wall thickening in CPP female rat. SXF declined FSH, LH, PRL and E2 concentration in peripheral blood in CPP female rat (P < 0.01or P < 0.001). SXF suppressed the expression of GnRH, GnRHR, ERα and GPR30 in hypophysis, the expression of GnRH in hypothalamus and GnRHR in ovary of CPP female rat (P < 0.05, P < 0.01 or P < 0.001).Conclusions: SXF had effective therapeutic effects on CPP female rat, which was worthy of promotion clinically.