Development of a new Collateral Cleavage-independent CRISPR/Cas12a based easy detection system for plant viruses

Ashish Srivastava,Taruna Gupta,Sonal Srivastava,Sunny Dhir,Prashant Kumar,Tripti Singhal,Asha Rani,Narayan Rishi

Journal of Virological Methods（2022）

引用 3|浏览3

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摘要

•CRISPR/Cas12 based viral detection gain popularity during the COVID-19 pandemic.•All the Cas12 and Cas13 based diagnostic methods are associated with collateral cleavage of single stranded probes.•To minimize the cost of the detection, we introduce CCI method for diagnosis of plant virus genome.•This technique utilizes specific dsDNA binding dye for detection.

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关键词

Begomovirus,Geminivirus,Detection,Point-of-care testing,cpf1,Trans cleavage

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