Polymer-Peptide Conjugate Vaccine for Oral Immunization.

The copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, well-known as "click" reaction, is widely used in organic synthesis, medicinal chemistry, and polymer science for the conjugation of molecular entities of all sizes. In this protocol, B-cell epitope J8, derived from group A Streptococcus (GAS) M protein, and universal T-helper epitope PADRE were conjugated to poly(methyl acrylate) (PMA) to form a self-assembled nanoparticle vaccine candidate (PMA-P-J8). The vaccine construct was orally administered to mice in a single dose of 30 μg, resulting in the production of a high number of serum (IgG) and salivary (IgA) antibodies.