Neuroprotective effects of microRNA 124 in Parkinson's disease mice

Background: Recent studies have revealed the close correlation between microRNAs (miRs) and Parkinson's disease (PD). Here, we aimed to investigate the neuroprotective effect of miR-124 in a PD mouse model. Methods: MiR-124 expression in human plasma was detected by qRT-PCR. PD mouse model was established by stereotactic injection of 6-hydroxydopmine. Lentivirus were used to deliver and overexpress miR-124 and Axin1 into the substantia nigra. Multiple behavioral tests and oxidative stress assays were carried out to access the protective effect of miR-124 against PD. Western blot and luciferase assay were conducted to dissect the underlying molecular mechanisms. Results: MiR-124 expression was decreased in PD patients. Overexpression of miR-124 in PD mice could improve motor defects, ameliorate dopaminergic neurons loss, and reduce oxidative stress. Mechanistically, miR124 targeted Axin1 directly, and then attenuated PD progression via suppressing Axin1 and activating the Wnt/beta-catenin pathways in PD mice. Conclusion: MiR-124 is an important neuroprotective factor, which suppresses Axin1 and activates Wnt/beta-catenin signaling pathways in PD mice.