Disorder-to-order transition of Synaptobrevin-2: Tracing the conformational diversity of a synaptic SNARE protein

•Synaptobrevin-2 is unstructured in the absence of interaction partners.•Lipid membranes and SNARE proteins induce helix formation of Synaptobrevin-2.•Membrane binding is mediated by electrostatic interactions.•SNARE complex stability is accomplished by 15 hydrophobic and one ionic layers.