Miniaturized bioluminescence technology for single-cell quantification of caspase-3/7

•Diagnostics of deregulations of the mediators in apoptotic pathways is important for a wide range of pathologies.•Challenge for any protease fluorescent reporter is to achieve specificity.•Sufficient limit of detection and quantification for analyses of Caspases in single cells is reached.•Reliable evaluation of caspase can potentially serve as a high throughput diagnostics.