Facile method to incorporate high-affinity ACAT/SOAT1 inhibitor F12511 into stealth liposome-based nanoparticle and demonstration of its efficacy in blocking cholesteryl ester biosynthesis without overt toxicity in neuronal cell culture

•Encapsulation of ACAT inhibitor F12511 (Nanoparticle F) in stealth liposomes.•Nanoparticles are relatively stable when kept at 4 ºC for several months.•Nanoparticle F displays high potency as inhibitor in neuronal and microglial cells.•Nanoparticle F has minimal toxicity in primary mouse neurons.•This method is applicable to other ACAT inhibitors and hydrophobic compounds.