Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay

Stephanie Popping,Richard Molenkamp,Joachim D Weigel,Pim G N J Mutsaers,Jolanda C Voermans,Sander van Boheemen,Marc C Shamier,Reina S Sikkema,David F Nieuwenhuijse,Bas B Oude Munnink,Marion P G Koopmans,Jeroen J A van Kampen

Journal of Virological Methods（2022）

引用 3|浏览19

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摘要

•SARS-CoV-2 variants can result in PCR target failure shown by CT-value discrepancy.•Multiple targets are key to provide and maintain reliable molecular diagnostics.•Genomic SARS-CoV-2 surveillance must also include primer and probe targeting areas.•Public release of primer/probe sequences are crucial to maintain PCR quality.

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关键词

SARS-CoV-2,PCR amplification,Molecular diagnostics SARS-CoV-2,ORF1ab mutations,Cobas,Dual-target assay

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