Vibrational characterization of the pesticide molecule Tebuconazole

•Tebuconazole is a systemic triazole fungicide with discussed carcinogenic effects.•Raman spectroscopy provides unique and detailed structural fingerprint(s) of the molecule(s) what makes it an ideal technique to study tebuconazole-biomacromolecule(s) interaction(s).•Tebuconazole possesses rich vibrational features generating also strong SERS signal to enable the detection of low concentrations of the molecule.•SERS spectroscopy is a potential analytical tool for selective and sensitive pollutants detection.