Production And Characterization Of Vitamin B-12 From Staphylococcus Sp. And Its Antimicrobial Activity

Vitamin B-12 is the major dietary supplementary for most animals and has widespread applications in healthcare. The present study deals with the isolation of vitamin B-12 producing strains from food waste soil samples. Three strains (VS1, VS2 and VS3) were isolated from the soil sample and the extraction was done using potassium ferrocyanide along with PBS buffer. Vitamin B-12 was exhibited at about 114.1g/L from VS1 strains than the VS2 (11.4g/L) and VS3 (22.88g/L).Also, the antimicrobial activity of the VS1 strain showed maximum resistance compared to VS2 and VS3 strains against pathogens. Finally, the 16s rRNA sequencing was performed for VS1 and VS3 strains and they were found to be Staphylococcus pasteuri and Staphylococcus hominis respectively. It can also be used as an additional diet enhancement for the treatment of Pernicious anemia.