Genkwanin Attenuates Lung Cancer Development By Repressing Proliferation And Invasion Via Phosphatidylinositol 3-Kinase/Protein Kinase B Pathway

Lung cancer is a primary threat to human life. Although diagnosing and treating lung cancer has improved with medicine development, the patient outcomes are still not satisfactory. Genkwanin is a natural flavonoid compound with significant anticancer value, but its effect on lung cancer is currently unknown. This research explores Genkwanin effects on lung cancer cells and the underlying mechanism. Different Genkwanin concentrations (20, 40, and 80 mu M) were used on A549 and H69AR cells. MTT test was used to detect proliferation. Transwell test detected migration and invasion levels. Western blot and qRT-PCR experiments measure PI3K/Akt protein and mRNA levels, respectively. Genkwanin inhibited the proliferation, migration, and invasion of A549 and H69AR cells by inhibiting the PI3K/Akt pathway. Further experiments showed no synergistic effect when Genkwanin was combined with a PI3K/Akt inhibitor. Concluding, Genkwanin inhibits lung cancer proliferation, migration, and invasion by inhibiting PI3K/AKT pathway suggesting an alternative therapy for tumor growth and metastasis.