Headspace Solid-Phase Microextraction Coupled With Gas Chromatography-Mass Spectrometry (Hs-Spme-Gc-Ms) For The Characterization Of Cigar Leaves

Manufacturers and consumers are increasingly concerned about high-quality cigar products with clear geographical sources. To characterize the chemical profiles of different geographical origins of cigar leaf samples, headspace solid phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) combined with a chemometrics method was used in this study. The chromatography and chemical composition of cigar leaf samples from 16 geographical origins were acquired and profiled. The chemical markers contributing to the differentiation of cigar leaf samples were observed and characterized by a supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) method of chemometrics. Eight chemical markers were tentatively identified to be used as specific chemical markers for the differentiation of geographical cigar leaf samples. HS-SPME-GC-MS method coupled with chemometrics analysis has potential to be used for discriminating different geographical origins for cigar leaf samples.