Indian Squid Ink Nano-Suspension: Formulation, Characterization, In Vitro Anti-Tumor Activity Against Human Breast Cancer And Human Kidney Epithelial Cell Lines

Cancer is one of the leading causes of adult deaths worldwide. In India, the International Agency for Research on Cancer (IARC) estimated indirectly the death of people from cancer. Almost 60% of drugs approved for cancer treatment are of natural origin. In this study we have developed different formulations of Indian squid ink extract nanosuspension from squid fish (namely F1, F2 and F3) and characterized for its various physiochemical evaluations and investigated its anti-tumour effect in human breast cancer cell lines (MCF- 7) and kidney epithelial (Vero) cell lines.The result revealed that the formulation F3 showed the better nano-scale particle size in the range of 87-125nm, better zeta potential of -21.2mV and in vitro drug release of 98.18 +/- 0.2 for 6 hours. The in vitro antitumor study indicated that the F3 formulation of Indian squid ink nanosuspension showed potential anti-tumour activity in a dose dependent manner against human breast cancer (MCF-7) cell line and human kidney epithelial (Vero) cell line compared with standard Indian squid ink extract. This study concluded that high pressure homogenization method could be suitable to formulate Indian squid ink nanosuspension (ISI-NS) with anti-tumour potential against MCF-7 and vero cell lines.