Lead Uptake And Localization In Glial Cell Cultures

Rola Barhoumi,Robert Taylor,Evelyn Tiffany-Castiglioni

METALS IN THE BRAIN: MEASUREMENT AND IMAGING（2017）

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摘要

This protocol describes the solubilization of Pb in stock solutions for treatment of cell cultures, its measurement by inductively coupled plasma mass spectroscopy (ICP-MS) as well as its localization in glial cell cultures by confocal fluorescence microscopy.

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关键词

Lead analysis, Astroglial cells, Cell culture, Inductively coupled plasma mass spectrometry, Fluorescence microscopy, Leadmium, Lysotracker, Mitotracker

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