Spectral Determination Of Mu(A), Mu(S) And G Of One Thick Turbid Sample From Three Scattered Light Signals

Development of cost-effective methods and instruments to accurately determine optical parameters of turbid materials can have wide applications in life science research and clinical applications such as blood test and assay of cell suspensions. We report a fast and photodiode based method without integrating spheres to measure three light scattering signals and determine absorption coefficient, scattering coefficient and anisotropy factor of the sample using only one optically thick sample. Results of optical parameters for microsphere suspension samples have been obtained from 460 to 1000nm and validated against the results predicted by Mie theory. Using a GPU executed Monte Carlo code and gradient decent based inverse algorithm, the inverse solutions for determination of optical parameters and their spectra can be achieved in real-time. The uniqueness of the inverse solutions has been proved.