Development Of A Quick And Easy Molecular Method For Differentiating Haemophilus (Glasserella) Parasuis From Species Belonging To The Genus Actinobacillus

A quick, easy, and economical molecular method, which is capable of differentiating Haemophilus (Glasserella) parasuis from several species belonging to genus Actinobacillus, is reported in this paper. These species are currently isolated from porcine airways, and show similar growth characteristics to those of H. (G.) parasuis. The proposed method involves a rapid extraction of RNA from the bacteria grown on agar plates followed by an agarose gel electrophoresis, where an unusual band pattern of the ribosomal RNA of H. parasuis can be seen. In this way, the 23S ribosomal RNA subunit is split into two bands, while the 23S ribosomal RNA subunit remained without splitting in the species belonging to the genus Actinobacillus.