Voltammetry Of Cholesterol And Its Precursor 7-Dehydrocholesterol On Boron Doped Diamond Electrode

In this study a novel approach for simultaneous determination of cholesterol and its precursor 7-dehydrocholesterol by differential pulse voltammetry on boron doped diamond electrode was developed. They are oxidizable in mixed media of acetoonitrile and strong acid exhibiting volrammetric peaks at potentials of ca +0.8 V and +1.1 V (7-dehydrocholesterol) and +1.45 V cholesterol (vs. Ag/0.0 mol l(-1) AgNO3/l mol.l-1 NAClO(4)). Detection limits in micoromolar concentration range were obtained for both compounds, DP voltanvmetry was applied on their determination in milk cream arter liquid-liquid extraction standard addition method.