Three Dimensional Characterization Of The Dentin Porous Network Using Confocal Laser Scanning Microscopy (Clsm)

In this paper, the 3D-morphology of the porous structure in dentin is investigated by confocal laser scanning microscopy (CLSM). The porous microstructure near the dentino-enamel junction (DEJ), which consists of tubules partly connected by lateral branches, was found to exhibit a complex geometry. We revisit and challenge previous 2D studies by focusing on the 3D morphology (tubule and branching geometry) and quantification of porosity. Our work provides fundamental insight into the microstructure of dentin which could be used to study the effect of age and pathologies. Furthermore, such information is essential for the design of biomaterials used in dentistry, e.g., to ensure more efficient bonding to dentin. Finally, our analysis of the tubular network structure provides valuable data that could be used directly as inputs in a numerical model.