Multimodal High-Resolution Embryonic Imaging With Light Sheet Fluorescence Microscopy And Optical Coherence Tomography
OPTICS LETTERS(2021)
摘要
A high-resolution imaging system combining optical coherence tomography (OCT) and light sheet fluorescence microscopy (ISFM) was developed. ISFM confined the excitation to only the focal plane, removing the out of plane fluorescence. This enabled imaging a murine embryo with higher speed and specificity than traditional fluorescence microscopy. OCT gives information about the structure of the embryo from the same plane illuminated by LSFM. The co-planar OCT and LSFM instrument was capable of performing co-registered functional and structural imaging of mouse embryos simultaneously. (C) 2021 Optical Society of America
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