Nmr Spectroscopy As A "Green Analytical Method" In Metabolomics And Proteomics Studies

Nuclear magnetic resonance (NMR) is one of two methods (in addition to MS) that predominate in metabolomics and structural analysis. Due to the great demand for this type of research, there is a very high consumption of solvents. Fortunately, most biological studies can be performed using simple protocols that include 10% D2O and 90% H2O. More complex metabolomics studies with polar compound extraction require the use of MeOH or MeCN as reagents for protein precipitation. The most extensive Folch extraction method requires a mixture of solvents (MeOH/H2O/CHCl3) for tissue metabolites and sometimes biofluid extraction. In this review, we show that NMR can be a green method for most biological studies that yields useful information where the overall approach is in line with the GSK (GlaxoSmithKline) solvent sustainability guide.